Lect. and lab. Presents purification methods, mass transfer coefficients, problems specific to biologicals, and scale-up of processes. Also covers chromatography, phase extraction, supercritical fluids, sedimentation, precipitation, electrophoresis, dialysis, affinity techniques, cell separation, application of separations to bioreactors, and comparison of batch and continuous processes. Prereq., senior standing or above in engineering or science. Same as CHEN 5820. Prerequisites: Requires pre-requisite course of CHEN 3220.